EPIC ORDER CODE LAB877 Culture, Acid Fast, includes Smear
Additional Codes
Sunquest Order Code: CAFB
Alias: Acid-Fast Bacilli (AFB), AFB (Acid-Fast Bacilli), Bacillus, Acid-Fast, Culture, TB (Tuberculosis), MTB (Mycobacterium tuberculosis), Mycobacteria Culture, Mycobacterium tuberculosis (MTB), TB (Tuberculosis), TB (Tuberculosis) Culture, Tubercle Bacilli: Mycobacterium tuberculosis, Tuberculosis (TB)
SPECIMEN
Collection Information: Sputum, Bronchoalveolar Lavage, Gastric Aspirate, Urine, Body Fluid, Tissue/Sterile Container.
Transport Information: Gastric lavage specimens must be treated with equal parts 1% sodium bicarbonate prior to transport to reference laboratory.
To prepare 1% sodium bicarbonate from a 7.5 % solution:
- Dilute 1.0 mL of 7.5% sodium bicarbonate with 13.0 mL of sterile deionized water.
- Mix
- Solution is stable 24 hours refrigerated.
Remarks: Aseptic technique must be used in obtaining, and transport of specimen.
See Specimen Collection Guidelines for specific specimen. Identification and susceptibility tests are billed separately from culture. SWABS ARE NOT RECOMMENDED FOR THE ISOLATION OF MYCOBACTERIUM, SINCE THEY PROVIDE LIMITED SPECIMEN MATERIAL.
For patients with clinical and chest x-ray findings compatible with tuberculosis, three sputum samples should be collected over an 8-24 hour period of time (including at least one first morning specimen).
Unacceptable Conditions: Dry specimen, Leaking container, inadequate volume of sputum or fluid specimens (<5 ml), specimen on swab.
SPECIMEN STABILITY
Refrigerated Stability: 7 days
Ambient Stability: 24 hours
CPT
87116; 87206; 87015
TEST METHOD
Tube Culture/ ESP continuous Monitoring System/ AFB Smear
TESTING LABORATORY
Pathology Laboratory
Laboratory Section: Microbiology
ANALYTICAL TIME
Performed: Setup daily, on day shift. (Cultures read on the dayshift only.)
Analytical Time: Smear results 24 hours, Culture 8 weeks